Anti-ATP6V1A Antibody

Product Name

Anti-ATP6V1A Antibody

View all ATP6V1A Antibodies

SKU/Catalog Number

A10401

Size

100ul

Form

Liquid

Description

Boster Bio Anti-ATP6V1A Antibody catalog # A10401. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ATP6V1A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10401)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human MRPL12.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A10401 is reactive to ATP6V1A in Human, Mouse, Rat

Applications

A10401 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

68.304kDa

Background of ATP6V1A

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex.

Marty L., J. Biol. Chem. 271:11468-11476(1996).
Liu J., Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team; Genome Res. 14:2121-2127(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:2000-1:5000
IHC: 1:50-1:200

Validation Images & Assay Conditions

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Figure 1. Western blotting validation for Anti-ATP6V1A Antibody A10401

Western blot analysis of extracts of various cell lines

Gene/Protein Information For ATP6V1A (Source: Uniprot.org, NCBI)

Alternative Names

70kD, isoform 1; ATP6A1; ATP6V1A1ATPase, H+ transporting, lysosomal, subunit A1; ATPase, H+ transporting, lysosomal (vacuolar proton pump), alpha polypeptide; ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A; EC 3.6.3; EC 3.6.3.14; H(+)-transporting two-sector ATPase, subunit A; H+-transporting ATPase chain A, vacuolar (VA68 type); HO68; VA68; vacuolar ATP synthase catalytic subunit A, ubiquitous isoform; Vacuolar ATPase isoform VA68; vacuolar proton pump alpha subunit 1; Vacuolar proton pump subunit alpha; V-ATPase 69 kDa subunit 1; V-ATPase 69 kDa subunit; V-ATPase A subunit 1; V-ATPas ATP6V1A ARCL2D, ATP6A11, DEE93, HO68, IECEE3, VA68, VPP2, Vma1, ATP6V1A ATPase H+ transporting V1 subunit A V-type proton ATPase catalytic subunit A|ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A|ATPase, H+ transporting, lysosomal, subunit A1|H(+)-transporting two-sector ATPase, subunit A|H+-transporting ATPase chain A, vacuolar (VA68 type)|V-ATPase 69 kDa subunit 1|V-ATPase A subunit 1|V-ATPase subunit A|vacuolar proton pump alpha subunit 1|vacuolar proton pump subunit alpha

For more info on ATP6V1A, check out the ATP6V1A Infographic

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In this infographic, you will see the following information for ATP6V1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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